These findings demonstrate the validity and feasibility of measuring I-FABP, but not transferrin, using DBS sampling. We found a weak linear association between plasma and DBS measurements of transferrin concentrations, but a strong linear association and high level of agreement between plasma and DBS measurements of I-FABP concentrations. Whole blood was used to create additional DBS samples and then spun down to plasma for analysis.

Matched DBS from a fingerstick and venous whole blood samples from n=74 individuals were collected over a 3-week period. We present a validation study of two DBS protocols for the measurement of transferrin, a biomarker of iron status, and I-FABP, a biomarker of intestinal permeability. Dried blood spot (DBS) biomarker sampling offers a minimally invasive method of detecting EED. Our objective is to provide investigators with the information they need to make informed decisions regarding the appropriateness of blood spot methods for their research interests.Д®nvironmental Enteric Dysfunction (EED) is a subclinical condition characterized by increased intestinal permeability and impaired nutrient absorption, but current methods for assessing EED are invasive and resource intensive. We present the results of a comprehensive literature review of published protocols for analysis of DBS samples, and we provide more detailed analysis of protocols for 45 analytes likely to be of particular relevance to population-level health research. Disadvantages include requirements for assay development and validation as well as the relatively small volumes of sample. Advantages of using DBS samples-compared with venipuncture include the relative ease and low cost of sample collection, transport, and storage. After a brief discussion of biomarkers more generally, we review procedures for collecting, handling, and analyzing DBS samples.

In particular using dried blood spot (DBS) samples-drops of whole blood collected on filter paper from a simple finger prick-provides a minimally invasive method for collecting blood samples in nonclinical settings. However recent methodological developments have overcome many of these constraints and have also expanded the options for incorporating biomarkers into population-based health research in international as well as domestic contexts. Logistical constraints associated with the collection and analysis of biological samples in community-based settings have been a significant impediment to integrative, multilevel bio-demographic and biobehavioral research.

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